Recent Advances Increasing the Capacity of TDNA

Another early problem with Agrobacterium-mediated transformation was the inability to transfer large DNA fragments to the plant genome. Although a maximum size limit for T-DNA gene transfer was not determined, the transfer of foreign DNAs larger than 30 kbp was not routinely achievable until about five years ago. This prevented the transfer of large genes or T-DNA sequences containing multiple genes. The problem was addressed by Hamilton and colleagues, who developed a novel binary vector system in which the T-DNA vector was based on a bacterial artificial chromosome and the vir component expressed higher levels of virG and virE. This bibac (binary BAC) vector facilitates the transfer of up to 150 kbp of DNA to the plant genome, allowing the introduction of gene libraries into plants and the simultaneous introduction of many genes on a cointegrate vector (34).

Was this article helpful?

0 0
Detox Diet Basics

Detox Diet Basics

Our internal organs, the colon, liver and intestines, help our bodies eliminate toxic and harmful  matter from our bloodstreams and tissues. Often, our systems become overloaded with waste. The very air we breathe, and all of its pollutants, build up in our bodies. Today’s over processed foods and environmental pollutants can easily overwhelm our delicate systems and cause toxic matter to build up in our bodies.

Get My Free Ebook


Post a comment